El cultivo de protoplastos en cítricos y su potencial para el mejoramiento genético. Article (PDF Available) · July with 1, Reads. Export this citation. Results 80 – 90 Download Citation on ResearchGate | On Jan 1, , R.R. Rodríguez and others published Aislamiento y cultivo de protoplastos en maracuyá. Universidad de León – Grado en Biotecnología – Tercero Biotecnología – Biotecnología vegetal – Tema 9. Cultivo de protoplastos e hibridación somática.
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Analysis of intra-specific somatic hybrids of potato Solanum tuberosum using simple sequence repeats.
Identification of somatic hybrids is a requisite for the effective exploitation of the protoplasts fusion in potato improvement. Root tips were stained by lacto-propionic orcein.
Cultivo y fusion de protoplastos 
What students say about Stuvia. Interspecific hybridization between the cultivated potato Solanum tuberosum subspecies tuberosum L. Then, at fifty days, all culture media were replaced by a mOsm V-KM medium. Production of somatic hybrids prottoplastos electrofusion in Solanum. Avoid resits and achieve higher grades with the best study guides, textbook notes, and class notes written by your fellow students.
Tema 9. Cultivo de protoplastos e hibridación somática
Interspecific somatic hybrids were obtained by polyethylene glycol fusion of protoplasts from tetraploid Solanum tuberosum L. Protoplast-fusion derived Solanum cybrids: New York, NY Molecular genetic characterization of plant somatic hybrids. When colonies had developed, alginate beads were despolimeryzed using 20 mM sodium citrate solution in sorbitol 0,3 M, pH 7,4 and gently shaking.
Non-transgenic applications of plant tissue culture in potato. Segundo curso Primer curso. After that, colonies were dispersed into plates with solid MS13K regeneration medium Benke, The enzyme treatment was carried out overnight with gentle agitation.
Successful application of the fusion technique demands a protocol for plant regeneration from protoplasts. Production and molecular characterization of Citrus intergeneric somatic hybrids between red tangerine and citrange.
Somatic hybrids were confirmed morphologically under greenhouse conditions, when intermediate characteristics between parent lines, such as shoot coloration, leaf shape and pubescence were observed. Isolation of plant DNA from fresh tissue.
After seven days, the culture medium was diluted 1: These bands also appeared in the fusion regenerants Figs. Cytological and RAPD marker analysis confirm their hybrid nature. Plant Cell Rep 3: It has been demonstrated that protoplast-derived plants show a higher degree of chromosomal number variation than those derived from tissue or organ culture. Preview 1 out of 20 pages. Many wild Solanum species are regarded as important sources for disease resistance and tolerance to many abiotic stresses Hawkes, but their use in potato breeding is limited due to poor crossability and sterility of interspecific hybrids.
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AISLAMIENTO y CULTIVO DE PROTOPLASTOS EN MARACUYA by juank quintero on Prezi
Abstract Interspecific somatic hybrids were obtained by polyethylene glycol fusion of protoplasts from tetraploid Solanum tuberosum L. Transfer of resistance to potato leaf roll virus from Solanum brevidens into Solanum tuberosum by somatic fusion.
Overspecific notes are at your disposal. These barriers in classical breeding can be overcome using biotechnological methods such protoplasyos somatic hybridization by protoplast fusion Carputo et al. With their assistance, you can become well prepared and can avoid having to retake exams. A slot-blot hybridization method for screening somatic hybrids.
Cultivo y fusion de protoplastos
What do you want to do? Improvements in regeneration from protoplasts of potato and studies on chromosome stability. Further work is needed to evaluate their resistance level to Phytophthora infestans Mont. These results showed that all hybrids were aneuploids Fig.
Nineteen somatic culttivo plants were obtained from fusion experiments using PEG in an effort to combine elite traits from both parents. Acknowledgments The authors would like to thank the International Potato Center for financial support and to Martha Williams de Castro who revised the English version of this article.
RAPD and chromosomal analysis of the hybrids are also presented. To select hybrids, regeneration of in vitro plants was subjected to RAPD analysis. Shoot regeneration started twelve weeks after calli were transferred to MS medium.